Dual Effect of Aldicarb on Acetyl Cholinesterase and Monoamine Oxidase in Different Parts of Rat Brain

Dual inhibition of acetyl cholinestrase (AChE) and mono amine oxidase (MAO) types A and B in whole brain, cerebellum, basal ganglia, frontal cortex, pons and medulla oblongata of rat brain by aldicarb (2‐methyl‐2(methylthio) propionaldehyde O‐methyl(carbamoyl) oxime (C 7 H 14 O 2 NS) in vitro was studied. The inhibition of AChE and MAO types A and B in different parts of the brain was found to be of competitive type. The highest values of the enzyme inhibitor dissociation constant (Ki) were obtained with the extracts of pons and medulla oblongata, these parts responsible for vital centers (e.g. respiration). In case of MAO‐A the highest Ki values were obtained with the extracts of the cerebellum and frontal cortex, these parts responsible for balance and perception respectively. In case of MAO‐B the highest Ki value was obtained with the extract of basal ganglia, the part responsible for sense. The inhibition of the enzyme extracts by aldicarb was reversible, and the enzymes restored their whole activities by dialysis. The dual inhibition of AChE and MAO types A and B by the carbamate aldicarb save the neurotransmitters (e.g. acetylcholine and the biogenic amines) in the tissues especially in the nervous tissues, which are of great importance for Alzheimer dementia patients (AD).