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Protective effects of fucoidan against UVB‐induced aging in human skin fibroblasts
Author(s) -
Lee Yong Hwan,
Moon Hee Jung,
Lee Soon Rye,
Jeong Seok Hoon,
Chang Hee Kyung,
Stonik Valentine A,
Rasskazov Valery A,
Zvyagintseva Tatiana
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb21-b
Subject(s) - fucoidan , photoaging , matrix metalloproteinase , chemistry , mtt assay , skin aging , human skin , pharmacology , viability assay , in vitro , polysaccharide , biochemistry , medicine , biology , dermatology , genetics
Long terms and repeated exposure of UVB light on the skin induces up‐regulation of matrix metalloproteinases (MMPs). UV‐induced MMPs cause connective tissue damage and skin to become aged. The present study was performed to elucidate the anti‐aging actions of fucoidan on UV‐induced aging in human skin fibroblasts. The HS68 cells were pretreated with fucoidan at doses of 10μ/ml, 25μ/ml, 50μ/ml for 24 h. After UVB irradiation, they were then further incubated. The level of aging were determined by measuring MTT assay, senescence‐associated ß‐galactosidase (SA‐ ßG) activity, and ELISA tests. In case of MTT assay, fucoidan treatment showed significant improvement in cell viability in the following order; 50μ/ml>25μ/ml>10μ/ml>100μ/ml(p<0.05). In ELISA test, pretreatment with fucoidan as doses of 10μ/ml, 25μ/ml, 50μ/ml significantly inhibited the production of MMP‐1 compared to the UVB only irradiated by 27%, 30%, and 34%, respectively (p<0.05). Additionally, SA‐ ßG activity was markedly decreased in the presence of fucoidan. Based upon these results, it seems that fucoidan might play an important role in the cellular defense mechanism against UV radiation‐induced photoaging. ∗This work was supported by the grant of KOSEF.