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Investigating pathogenic protein interactions with heparin and heparan sulfate using surface plasmon resonance
Author(s) -
Kemp Melissa M,
Zhang Fuming,
Linhardt Robert J
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb21-a
Subject(s) - heparan sulfate , heparin , surface plasmon resonance , glycosaminoglycan , chemistry , listeria monocytogenes , microbiology and biotechnology , biochemistry , protein–protein interaction , biophysics , bacteria , biology , nanotechnology , nanoparticle , materials science , genetics
Heparin and heparan sulfate glycosaminoglycans are two polysaccharides that are important in almost every aspect of life. Their interaction with various proteins facilitate many vital biologically processes. However, they can also be exploited by various pathogens causing devastating consequences. Many viruses, bacteria, and parasites contain proteins on their outer surface that are able to interact with heparin and heparan sulfate leading to pathogen invasion. The interaction between heparin and heparan sulfate and proteins from three different pathogens, internalin B (InlB) from Listeria monocytogenes, alpha C protein (ACP) from group B Streptococcus, and circumsporozoite protein (CSP) from Plasmodium falciparum , were examined with surface plasmon resonance to obtain kinetic and affinity data. The interactions between these molecules were complex and did not exhibit a simple bimolecular interaction. Characterization of the interaction between these proteins and glycosaminoglycans helps explain the ability of these pathogens to infect host cells. This research is funded by NIH.