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Stability of the Hypoxia inducible factor 1α ‐ promoter complex in vivo
Author(s) -
Yu Peng,
Kodadek Thomas
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb18
Subject(s) - promoter , chromatin immunoprecipitation , hypoxia inducible factors , immunoprecipitation , gene , transcriptional regulation , chemistry , transcription factor , dissociation (chemistry) , hypoxia inducible factor 1 , microbiology and biotechnology , gene expression , biology , genetics
The dynamics of the association and dissociation of transcriptional factors to and from their promoters plays critical roles in determination of the gene expression profiles in eukaryotes. Recent studies showed that the lifetime of different transcriptional factor‐promoter complexes can vary widely. Using a variant of the chromatin immunoprecipitation assay that allows direct observation of the stability of such complexes(Nalley, K., Johnston, S.A., & Kodadek, T. Proteolytic turnover of the Gal4 transcriptional factor is not required for function in vivo. Nature (2006) 442:–7), we find that the hypoxia inducible factor 1 α(HIF1α) is stable on its responsive elements under hypoxia conditions, having a half‐life longer than 1 hour. However, when the activation domain of the HIF1α is truncated, the half‐life of the complex decrease to about 5min, indicating the activation domain is required for the HIF1 to stay stably on the promoter.