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The role of Eserine and GABA in the activity of DMV on airway smooth muscle tone in ferrets
Author(s) -
Neziri Burim,
Krasniqi Shaip,
Jakupaj Muharrem,
Devaja Shqipe
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb124-c
Subject(s) - acetylcholine , stimulation , chemistry , cholinergic , contraction (grammar) , neurotransmitter , vagus nerve , gaba receptor antagonist , muscle contraction , physostigmine , choline acetyltransferase , pharmacology , bicuculline , anesthesia , neuroscience , endocrinology , biology , medicine , gabaa receptor , central nervous system , receptor , biochemistry
In this study, we examined effects of chemical stimulation of the dorsal motor nucleus of vagus (DMV) in respiration process. We observed that in anesthetized, paralyzed and artificially ventilated ferrets, DMV stimulation or inhibition is followed by accordingly airway smooth muscle contraction or relaxation. To clarify the mechanisms underlying this observations, we examined the effects of acetylcholine (ACh) as well as GABA in this group of neurons by: stimulation of DMV by eserine as and choline acetyltransferase (ChAT) inhibitor, which in terms increase the level of ACh in the synaptic junction; inhibition of this group of neurons (DMV) by administration of GABA as an inhibitor neurotransmitter. We have performed physiological experiments under in vivo conditions to determine the effects of stimulation or inhibition of DMV by eserine or GABA on the airway smooth muscle (ASM) tone that was manifested with an increase or decrease in pressure in tracheal segment (Ptseg), lung resistance (RL) or dynamic compliance (Cdyn). These studies indicate that DMV play a great role in regulating the respiration process by contraction or relaxation of ASM tone. Furthermore, these studies showed that in the region of DMV are present the GABAergic neurons as well as GABA receptors.