Premium
Interleukin‐6 response to shock wave lithotripsy‐induced renal injury
Author(s) -
Clark Daniel Lynn,
Handa R K,
Johnson C D,
Connors B A,
Evan A P,
Willis L R
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb123
Subject(s) - medicine , renal cortex , medulla , renal medulla , kidney , cortex (anatomy) , urology , anatomy , biology , neuroscience
Shock wave lithotripsy (SWL) produces a rapid inflammatory response in renal tissue. This study assessed the time course and location of the inflammatory response in our established porcine model of acute SWL‐induced renal injury, using interleukin‐6 (IL‐6) as a marker of inflammation. The lower pole left renal calyx of anesthetized female pigs (30–35 lbs) received 2000 shock waves (SW) from a Dornier HM3 lithotripter (24 kV, 120 SW/min). Blood and urine samples were taken at timed intervals. At 4 hours post‐treatment, kidneys were perfused with cold saline, excised, and tissue samples were flash‐frozen in liquid N 2 . IL‐6 was measured by ELISA (R&D Systems). IL‐6 was not detectable in all blood, urine and renal tissue (cortex and medulla) samples from control animals (n=5). In SWL‐treated animals (n=6), measurable levels of IL‐6 were found in the SWL‐treated lower pole medulla (18 ± 9 pg/mg protein, n=3). In contrast, blood, urine and other renal tissue (contralateral cortex and medulla; treated kidney: lower and upper pole cortex, upper pole medulla) samples from SWL‐treated animals did not contain IL‐6. In conclusion, while IL‐6 is not a good blood or urinary marker for acute SWL‐induced renal injury, elevated tissue levels of IL‐6 following SWL appear to be highly localized to those medullary regions directly exposed to SWs, which are also sites of significant SWL‐induced tissue trauma. Supported by NIH grant DK67133.