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Fusogenic lipid vesicles loaded with adenosine triphosphate (ATP) deliver energy to rat skeletal muscle
Author(s) -
PerezAbadia Gustavo,
Arumugam Sengodagounder,
Vasilic Dalibor,
Goga Ledia,
Lane Andrew N,
Barker John H,
Maldonado Claudio
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb117
Subject(s) - phosphocreatine , adenosine triphosphate , atp hydrolysis , atp synthase , chemistry , high energy phosphate , skeletal muscle , myocyte , oxidative phosphorylation , adenosine diphosphate , biochemistry , energy metabolism , medicine , biophysics , biology , endocrinology , enzyme , atpase , platelet , platelet aggregation
In rat transplant studies, donor hindlimbs (HLs) perfused with a solution containing fusogenic lipid vesicles loaded with ATP (FLVs‐ATP) remained viable after 13h of warm ischemia and transplantation. Histological analysis of donor muscles 7 days post‐op revealed that myocytes were regenerating. It was not clear whether FLVs‐ATP were delivering ATP to skeletal muscle. The purpose of this study was to determine whether HLs perfused with FLVs‐ATP delivered ATP to myocytes. We hypothesized that if FLVs‐ATP delivered ATP intracellularly the hydrolysis rate of phosphocreatine (PCr) would be prolonged in treated muscles. To test this hypothesis phosphate spectra from HL muscles were analyzed using 31 Phosphate Nuclear Magnetic Resonance ( 31 P NMR). Rat HLs were allocated into 6 groups (n=10/group) and perfused with one of the following solutions: Heparinized Lactated Ringer’s (HLR); ATP only 5mM and 10mM; empty FLVs (no ATP); FLVs‐ATP 10mM and 20mM. 31 P NMR spectra were recorded for 3 h, and PCr half‐life was calculated using peak intensity values. The PCr half‐life of muscles treated with FLVs‐ATP 20mM (16.9 ±0.7 min) and ATP 10mM (18.2 ±0.8 min) was significant (p<0.05) when compared to both HLR (13.7 ±0.8 min) and empty FLVs (6.1±2.2 min). Means for HLR and empty FLVs were significantly different (p<0.05). We conclude that both FLVs‐ATP and free ATP had a PCr sparing effect, however, the mechanisms are not clear.