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Interleukin‐15 does not attenuate TNF‐alpha and/or aging induced pro‐apoptotic signaling in skeletal muscles
Author(s) -
Pistilli Emidio Edward,
Siu Parco M,
Alway Stephen E
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb116-b
Subject(s) - apoptosis , skeletal muscle , dna fragmentation , tumor necrosis factor alpha , in vivo , endocrinology , medicine , myocyte , biology , chemistry , programmed cell death , biochemistry , microbiology and biotechnology
In this study, we tested the hypothesis that IL‐15 would attenuate apoptosis induced by TNF‐alpha in vitro and/or aging in vivo, specifically in skeletal muscles. Treatment of C2C12 myoblasts with 20ng.ml‐1 of TNF‐alpha decreased cell viability and reduced mitochondrial integrity. However, treatment with 20ng.ml‐1 of IL‐15 did not attenuate the TNF‐alpha induced decrease in cell viability and mitochondrial integrity, indicating IL‐15 failed to protect against TNF‐alpha induced apoptosis in vitro. To test the effects of IL‐15 on muscle in vivo, IL‐15 (100ìg.kg‐1 per day) was administered via osmotic pumps, to young adult (n=6) and aged (n=6) Fischer344 x Brown Norway (FBN) rodents for 14d. DNA fragmentation was greater in plantaris, soleus, and medial gastrocnemius muscles from aged animals and in all muscles from IL‐15 treated animals compared to muscles from control animals. IL‐15 had minimal effects on the incidence of DNA fragmentation in left ventricle, liver, or spleen of experimental rodents. These data do not support our initial hypothesis and suggest that IL‐15 does not prevent apoptotic signaling in induced by TNF‐alpha and in muscle tissue from aged rodents. The proposed anti‐apoptotic property of IL‐15 may be tissue specific and/or specific to the degree of pathology; however, additional research is required to more clearly decipher this role. Supported by: R01 AG‐021530 (Alway), RFG‐14 (Pistilli)