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Differential expression of SIX1 in humans and murine muscle culture
Author(s) -
Kostek Matthew Christopher,
Rennie Michael,
Cuthbertson Daniel,
Shi Rongye,
Fedele Mark,
Esser Karyn,
Chen YiWen
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb108-b
Subject(s) - transfection , c2c12 , myogenesis , gene expression profiling , biology , gene , cell culture , expression vector , myocyte , microbiology and biotechnology , gene expression , skeletal muscle , endocrinology , genetics , recombinant dna
To elucidate differences between muscle lengthening (L) and shortening (S) contractions we compared changes in gene expression, using temporal expression profiling, within 24h of an acute bout of each type of contraction conducted simultaneously in 5 healthy young men. Biopsies were taken before exercise and 3, 6, and 24h afterwards. Expression profiling analysis revealed only two genes as differentially expressed between the two types of contractions at more than one time point. One of these genes, Sine Oculis Homeobox 1 (SIX1), is expressed primarily in adult skeletal muscle. Expression was verified using qRT‐PCR (L/S, 3h) ‐1.9 fold, P < 0.001. To examine the affect of this gene in myogenesis, we transfected (Fugene6) C2C12 cells with a vector containing murine Six1 or a verified Six1‐RNAi sequence and the corresponding empty vector controls. Proliferation (cell count) was lower for the Six1‐RNAi transfected cells at 72h (P = 0.011) after transfection as compared to empty vector. No differences in fusion index were observed at 72h (P = 0.393) after transfection and incubation in differentiation medium (2% horse serum). This study suggests that Six1 is involved in muscle remodeling after L contractions.