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The modulation of antioxidant defences in response to maximal static apnea
Author(s) -
Bulmer Andrew,
Coombes Jeff S.,
Sharman James E.,
Ridgeway Lynne,
Stewart Ian B.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb106-b
Subject(s) - oxidative stress , trolox equivalent antioxidant capacity , antioxidant , superoxide dismutase , malondialdehyde , ferric reducing ability of plasma , catalase , chemistry , trolox , endocrinology , medicine , bilirubin , glutathione peroxidase , antioxidant capacity , biochemistry
Apneic behaviour can result in circulatory oxidative stress suggesting such practices could negatively affect human health. This study quantified the oxidative stress response in apnea trained and control subjects and determined changes in their antioxidant systems after five static apneas. Blood was taken at baseline (B) and after the first (A1), third (A3) and fifth (A5) apneas. Plasma antioxidant status was assessed using trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP) and bilirubin assays. The activity of RBC antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidise (GPx) were determined and plasma malondialdehyde (MDA) assessed oxidative stress. Plasma bilirubin decreased from baseline to A1 (controls −17.2%; divers −17.8%; P <0.01) as did FRAP (controls −3.2%, divers −3.2%; P <0.03). Significant group differences in TEAC were noted at A1 (controls 1.76±0.18; divers 1.91±0.09 MEqu/Trolox/L; P <0.05). From baseline to A5, apnea increased SOD (controls 11.3%; divers 11.8%; P <0.05) and GPx activity (controls 14.8%; divers 17.7%; P <0.04), but decreased CAT activity (controls −7.3%; divers −2.0%; P <0.03). Apnea did not affect MDA ( P >0.32). These data suggest there was no oxidative stress response to static apnea and that the modulation of antioxidant enzyme activity may be responsible for this protection.