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Embryonic Expression of Ion Channels and Exchangers Important for Gastric Acid Secretion
Author(s) -
Parker Lynz J,
Koehler James L,
Hinkle Karen L
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb101-a
Subject(s) - embryonic stem cell , biology , secretion , stomach , microbiology and biotechnology , gene expression , gastric acid , potassium channel , gene , endocrinology , biochemistry
Proper ion exchange across the parietal cell membrane is essential for normal secretion of gastric acid in the stomach. Several ion exchangers and channels have been identified that are essential for normal parietal cell function, including the HCO3/Cl‐ exchanger AE2 and the potassium channel KVLQT1. Genetically‐engineered mice deficient for either one of these factors have severe impairments in acid secretion; however, the patterns of gene expression during embryonic development have yet to be determined for Ae2 (Slc4a2) and Kvlqt1. The overall goal of this research was to analyze the timing of expression of Kvlqt1 and the Ae2 during mouse development. Using embryonic mouse RNA days 10 through 18, the patterns of gene expression for these factors were analyzed using non‐quantitative and quantitative RT‐PCR. Our results show that both Kvlqt1 and Ae2 are expressed starting at embryonic stage E11 and continuing through E18. This is not surprising because these genes are expressed in numerous tissues which may develop before the stomach. Further studies will be conducted using immunohistochemistry to specifically identify when KVLQT1 and AE2 are expressed in the stomach.