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INHIBITION OF MATRIX METALLOPROTEINASE ACTIVITY BY SELENIUM AND DOXYCYCLIN IN HEART AND AORTA OF DIABETIC RATS
Author(s) -
Koksoy Ayse Aslihan,
Sariahmetoglu Meltem,
OnayBesikci Arzu,
Schulz Richard,
Turan Belma
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb100-d
Subject(s) - matrix metalloproteinase , diabetes mellitus , streptozotocin , medicine , diabetic cardiomyopathy , aorta , doxycycline , selenium , endocrinology , pharmacology , cardiomyopathy , chemistry , heart failure , biochemistry , organic chemistry , antibiotics
Evidence from animal models suggests that reactive oxygen species play an important role in the development of diabetic cardiomyopathy. MMPs contribute to remodeling of the extracellular matrix after ischemia‐reperfusion, however, their role in myocardial dysfunction in type 1 diabetes is unknown. Current literature supports the role of selenium and doxycycline in reducing oxidant‐related tissue injury. We hypothesized that administration of doxycycline (Doxy; 15 mg/kg/day) or selenium (sodium selenate,Se; 3 mg/kg/day) may be protective for cardiovascular system in diabetes through MMP inhibition. Hearts and aortic vessels from streptozotocin‐induced (50 mg/kg) diabetic rats were used for analysis of MMP‐2, TIMP protein levels and MMP‐2 activity. Diabetes‐induced reduction in protein levels and activity of MMP‐2 in the aortic vessels and heart tissue and also the TIMP‐4 levels in the heart tissues from diabetic animals were restored back to near control levels with both Doxy and Se treatment. Supported by projects (TUBITAK‐SBAG‐3056) (Ankara University BAP20030809120)(TUBA‐young investigator award)