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Effect of beta‐lapachone on anti‐metastatic activities in hepatoma cell lines, HepG2 and Hep3B
Author(s) -
Kim Sung Ok,
Kwon Jae Im,
Kim DongEun,
Nam SooWan,
Choe WonKyung,
Choi Yung Hyun
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.lb10-b
Subject(s) - western blot , matrigel , cell culture , mtt assay , gentamicin protection assay , viability assay , gene knockdown , metastasis , microbiology and biotechnology , cancer cell , cell growth , biology , chemistry , cancer research , gene , cancer , biochemistry , genetics
β‐lapachone, a quinone is a compound obtained from the bark of the lapacho tree ( Tabebuia avellanedae ), was reported to have anti‐inflammatory and anti‐cancer activities. In this study, we investigated novel functions of β‐lapachone about anti‐metastasis and anti‐invasion using human hepatocarcinoma cell lines, HepG2 and HepG3. β‐lapachone dose‐dependently inhibited cell viability and migration of both HepG2 and Hep3B cells according to MTT assay and wound healing assay. RT‐PCR and Western blot data revealed that β‐lapachone dramatically induced the levels of protein as well as mRNA expression of early growth response gene‐1 (Egr‐1) and throbospondin‐1 (TSP‐1) on early time and then decreased in a time‐dependent manner. In addition, the down‐regulation of Snail and up‐regulation of E‐cadherin expression were observed in β‐lapachone‐treated HepG2 and Hep3B cells, which was associated with the decreased invasive ability as measured by matrigel invasion assay. Taken together, our results strongly suggest that β‐lapachone may be expected to inhibit progression and metastasis of hepatoma cells, at least in part, by inhibiting the invasive ability of the cells via up‐regulation of the expression of the Egr‐1, TSP‐1 and E‐cadherin.