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Mobilization of insoluble copper salts and minerals by methanobactin
Author(s) -
Zook James,
Behling Lee,
Hartsel Scott,
Dispirito Alan
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a998-a
Subject(s) - chemistry , methane monooxygenase , copper , stoichiometry , inorganic chemistry , extraction (chemistry) , malachite green , inductively coupled plasma mass spectrometry , mass spectrometry , environmental chemistry , methane , chromatography , adsorption , organic chemistry
Methanobactin (mb) is a chalkophore produced by Methylosinus trichosporium OB3b. It binds Cu(II) ions at a 1:1 stoichiometry very strongly (K d > 10–30 M) and many other ions with varying stoichiometries and affinities. It is produced in abundance under copper limiting conditions, and contributes to the activity of membrane‐bound methane monooxygenase. Since M. trichosporium is widely distributed in the environment and is used in bioremediation of hydrocarbons, it was of interest to see if mb could contribute to mobilization of potentially toxic metals in the environment. In experiments using atomic absorption, UV/visible spectroscopy, and inductively coupled plasma mass spectrometry (ICP‐MS) analysis, we find that mb can lead to a net extraction of Cu(II) from insoluble Cu(II)‐containing substances including Cu(II)oxide and Cu(II)‐containing minerals such as tetrahedrite and malachite.