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RT‐PCR Examination of Nervous System Contribution in Joint Inflammation.
Author(s) -
Jenkins Sarah Elizabeth,
Spears Robert
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a966
Subject(s) - neuropeptide y receptor , inflammation , medicine , endocrinology , neuropeptide , trigeminal ganglion , substance p , messenger rna , real time polymerase chain reaction , downregulation and upregulation , biology , neuroscience , biochemistry , receptor , sensory system , gene
Objectives: The goal of this study was to use RT‐PCR to examine changes in the expression of neuropeptide Y (NPY) during inflammation of the TMJ. Methods: Ten adult male Sprague Dawley rats were injected with complete Freund’s adjuvant (CFA) into the TMJ to induce inflammation. The rats were sacrificed 48 hours later along with ten age‐matched, uninjected controls. TMJ tissues, trigeminal ganglia (TG) and superior cervical sympathetic ganglia (SCG) were removed and homogenized. RNA was extracted and real time RT‐PCR was run to examine changes in RNA expression for NPY. Results: RT‐PCR results indicate that NPY mRNA was significantly increased in the inflamed TMJ tissues (p < 0.01) relative to the uninjected controls. However, while there was no significant difference in NPY levels between the inflamed and control TG, NPY mRNA levels were significantly increased in the SCG of CFA‐treated animals in comparison to the controls (p < 0.01). Conclusions: This study demonstrates that adjuvant‐induced inflammation of the TMJ resulted in an upregulation of mRNA expression of neuropeptide Y in inflamed TMJ tissues, and it appears that this increase is due to release from sympathetics, not the trigeminal system.