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Dietary Flavonoids and Renal Ion Transport
Author(s) -
Platt Laura K.,
Muchatuta Monalisa,
BlazerYost Bonnie L.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a955-a
Subject(s) - genistein , chemistry , apigenin , in vivo , epithelial sodium channel , nephron , reabsorption , quercetin , ion transporter , endocrinology , pharmacology , medicine , sodium , flavonoid , biochemistry , biology , microbiology and biotechnology , membrane , organic chemistry , renal function , antioxidant
Flavonoids are a class of compounds occurring in plants (e.g. parsley, cocoa, soybeans, tea), whose ingestion may be associated with lowered blood pressure by an unknown anti‐hypertensive mechanism. We examined the effects of three flavonoids, apigenin, genistein and quercetin, on renal electrolyte transport. The principal cells lining the distal nephron and cortical collecting duct are responsible for regulated ion transport. The mouse kidney cell line of the cortical collecting duct clone 4 (mpkCCD cl4 ) mimics the in vivo characteristics of the principal cell type, including high transepithelial resistance and hormonally responsive vectorial ion transport. Electrophysiological techniques can be used to screen for potential effects of nutritional elements such as flavonoids on electrolyte transport. In mpkCCD cl4 cells, a 30 minute treatment with genistein, apigenin, or quercetin inhibits transepithelial sodium transport. This ion transport is in an absorptive direction indicating that the agents may be inhibiting sodium reabsorption in vivo. Studies such as these have implications for understanding dietary influences on parameters responsible for the maintenance of normal blood pressure, as well as providing novel targets for treatment of high blood pressure. Funded by Indiana University – Purdue University Undergraduate Research Opportunities Program and a University College Leadership Grant