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Prolactin does not play a role in the down‐regulation of key lipogenic transcripts in major adipogenic tissues of periparturient rats exposed to hypergravity
Author(s) -
Patel Osman V.,
Ronca April,
Wade Charles E.,
Plaut Karen
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a950
Subject(s) - fatty acid synthase , medicine , endocrinology , lactation , prolactin , lipogenesis , lipid metabolism , biology , acetyl coa carboxylase , lipoprotein lipase , fatty acid synthesis , fatty acid , chemistry , adipose tissue , hormone , pyruvate carboxylase , enzyme , pregnancy , biochemistry , genetics
Demand for fatty acids in lactation repartitions lipid metabolism from adipose tissue (AD) and liver (LV) to mammary gland (MG). Prolactin (PRL) regulates Acetyl CoA carboxylase‐α (ACC) and fatty acid synthase (FAS) that are key to MG lipogenesis. Changes in gravitational load perturb PRL secretion and affect rat MG metabolism. We determined the expression of Lipoprotein Lipase (LPL), ACC and FAS genes in AD, LV and MG in stationary (ST, n=4) and hypergravity (HG) (n=5/group) exposed periparturient rats. Pregnant Sprague Dawley rats were exposed to 2‐g force from day 11 of gestation (G11) through post partum day 1 (P1). The HG rats were divided into untreated (UHG) or treated with PRL (HG‐PRL) or bromocryptine (HG‐BC) from G13 to P1. TaqMan® Assays‐on‐Demand TM kits were utilized for qPCR. All three genes were down‐regulated ( p <.01) in MG of the 3 HG groups compared to ST. LPL and FAS genes were repressed ( p <.01) in AD of UHG and HG‐PRL groups but not in HG‐BC compared to ST. ACC and FAS mRNA’s were suppressed ( p <.01) in UHG group compared to ST‐LV. HG suppress key enzymatic genes involved in lipid metabolism during lactation independent of PRL. (Supported by NASA NNA05CP91A)