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Leptin receptors in human skeletal muscle
Author(s) -
Guerra Borja,
Santana Alfredo,
Fuentes Teresa,
DelgadoGuerra Safira,
Socorro Alfredo Cabrera,
Dorado Cecilia,
Calbet José AL
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a942-b
Subject(s) - skeletal muscle , leptin , adipose tissue , western blot , gene isoform , medicine , endocrinology , receptor , leptin receptor , polyclonal antibodies , biology , antibody , biochemistry , gene , immunology , obesity
The aim of this study was to determine if leptin receptors (OB‐R) are expressed at the protein level in human skeletal muscle. Muscle biopsies were obtained from the musculus vastus lateralis in fourteen, healthy men (age = 33.1 ± 2 yr, height = 175.9 ± 1.7 cm, body mass = 81.2 ± 3.8 kg, body fat = 22.5 ± 1.9%). The expression of OB‐R protein was determined by Western blot in protein extracts prepared from muscle biopsies obtained from the musculus vastus lateralis, and also in subcutaneous adipose tissue obtained from the thigh using a polyclonal rabbit anti‐human leptin receptor recognising the human leptin receptor. The specificity of the anti‐OB‐R antibody was confirmed using competitive assays with a recombinant human peptide containing the extracellular domain of OB‐R (amino acid residues 1‐839). Three bands with a molecular weight closed to 170, 128 and 98 KDa were identified by Western blot with the anti‐OB‐R antibody. The 170 KDa band was detected only in skeletal muscle. The 128 and 98 KDa bands were identified in skeletal muscle and thigh subcutaneous adipose tissue. The 128 KDa isoform was not detected in four subjects, while in the rest the 128 KDa isoform measured was fully explainable by the presence of intermuscular adipose tissue. In summary, this study shows that a long isoform of the leptin receptor with a molecular weight close to 170 KDa is expressed at the protein level in human skeletal muscle.