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Increased ryanodine receptor type‐1 expression by Resistive Vibration Exercise (RVE) in human skeletal muscle fibers of 55‐days bed rest subjects
Author(s) -
Salanova Michele,
Püttman Britta,
Schiffl Gudrun,
Felsenberg Dieter,
Blottner Dieter
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a942-a
Subject(s) - ryanodine receptor , rest (music) , skeletal muscle fibers , skeletal muscle , medicine , endocrinology , resistive touchscreen , receptor , biology , computer science , computer vision
The expression of the intracellular calcium release channel RyR1 may be affected by muscle activity or inactivity. We tested the hypothesis whether in a voluntary male 56‐days horizontal bed‐rest (Berlin BedRest, 2003) RyR1 expression was affected by RVE, a short countermeasure protocol that is based on resistive mechanical load and reflexive neuromuscular stimuli. Needle biopsies were taken from vastus lateralis (VL) and soleus (SOL) of bedridden candidates of control (Ctrl) and, RVE group (each n=10) before and after bed rest. Samples were analyzed for RyR1 confocal analysis and [3H]‐ryanodine binding assay. Results from confocal clearly showed that Ctrl type‐I and ‐II fibers of both VL and SOL revealed different RyR1 staining patterns. Following bed rest without exercise RyR1 relative fluorescence intensity was two‐fold decreased in both myofiber types of post bed rest. After RVE, RyR1 relative fluorescence intensity was significantly increased in both type fibers of both muscles. By using [3H]‐ryanodine binding assay we confirmed the quantitative RyR1 changes in both VL and SOL following RVE. We conclude that RVE affects RyR1 expression in human VL and SOL. RVE should be implemented as an effective physiological countermeasure protocol against muscle atrophy and following prolonged clinical immobilization or long‐term human space‐flight missions. Sponsored by BMBF/DLR grants 50WB0145 & 50WB0521 to D.B.