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HSP70 in serum and mononuclear cells during heat acclimation in humans
Author(s) -
Amorim Fabiano Trigueiro,
Yamada Paulette,
Schneider Suzanne,
Moseley Pope
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a935-d
Subject(s) - hsp70 , peripheral blood mononuclear cell , western blot , heat shock protein , acclimatization , immune system , extracellular , heat shock , chemistry , andrology , downregulation and upregulation , immunology , medicine , endocrinology , biology , biochemistry , in vitro , gene , botany
Heat acclimation (HA) induces physiological changes that increase heat dissipation and exercise endurance. At the cellular level, heat exposure results in the accumulation of heat shock proteins (HSPs) which is associated with thermotolerance and downregulation of the inflammatory response. Conversely, extracellular HSP70 has been proposed to work as a signaling molecule activating the immune system. We wanted to understand the potential for extracellular HSP generation during heat conditioning, and so we chose to investigate changes of HSP70 in serum and mononuclear cells (MONO) during HA. Five subjects were heat acclimated 10 days. Serum HSP70 and MONO HSP70 were measured pre and post HA on days 1 and 10 by ELISA and Western blot, respectively. As expected, HA induced a reduction in heart rate and core temperature. MONO HSP70 increased from pre to post exercise on day 1 (1.0 ± 0.0 and 1.9 ± 0.7 unit p<0.05) but did not change from pre to post exercise on day 10 (1.8 ± 1.0 and 2.2 ± 0.7 unit). Serum HSP 70 increased from pre to post exercise on day 1 (0.7 ± 0.3 and 1.1 ± 0.4 ng/mL p<0.05) but did not change from pre to post exercise on day 10 (1.0 ± 0.4 and 1.0 ± 0.2 ng/mL). These results indicate that HA alters the cellular HSP70 response in MONO but also in serum HSP70, which can drive an inflammatory response. Thus, the process of HA results in both pro and anti‐inflammatory changes in the intact organism. Supported by DARPA W911NF‐06‐1‐0025