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The Role and Regulation mechanism of the phosphorylation of VASP in the Changes of Cytoskeleton Induced by ox‐LDL in Endothelial Cells
Author(s) -
Wang Nian,
Zhang Deling,
Wang Yinfang,
Ouyang Jingping,
Liu Yongming
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a914-c
Subject(s) - phosphorylation , protein kinase c , microbiology and biotechnology , cytoskeleton , actin cytoskeleton , actin , stress fiber , microfilament , chemistry , biology , cell , biochemistry , focal adhesion
The state and distribution of the cytoskeleton are intimately related to the endothelial integrity. Vasodilator stimulated phosphoprotein (VASP) is a crucial regulator of actin which is identified as main component of microfilaments in cytoskeleton. Activated PKC can induce VASP phosphorylation. It is reported that ox‐LDL can activate PKC pathway in endothelial cells through the specific receptor binding. In order to investigate the role of VASP phosphorylation in ox‐LDL‐induced changes of cytoskeleton in endothelial cell and identify whether PKC participates the phosphorylation of VASP induced by ox‐LDL, the endothelial cell line (ECV 304 ) was divided into 6 groups with different condition, which including the Control (normal culture), PKC activator(PMA 0.1μM), PKC inhibitor (GF109203X 1μM), ox‐LDL(200μM), PMA+ox‐LDL group, and GF109203X+ox‐LDL group. We observed that the formation of stress fiber increased in ox‐LDL, PMA and ox‐LDL+PMA groups, but actin clustered in the peripheral region of the cells decreased, while phosphorylated VASP localized to cell‐cell junctions and the lengthwise of actin cytoskeleton through confocal microscopy. The expression of phosphorylated VASP and PKC activity also increased in above groups, especially in ox‐LDL+PMA group. But the difference between GF109203X and Control group has no statistical significance. These results indicate that the phosphorylation of VASP mediates the changes of cytoskeleton induced by ox‐LDL in endothelial cells; PKC is one of the regulated kinases of the phosphorylation of VASP induced by ox‐LDL. The research is supported by “ChenGuang Youth Scientific Project of Wuhan” (20015005045).