Premium
Eutrophic structural remodeling of mesenteric resistance artery in Type 2 diabetic mouse
Author(s) -
Palen Desiree I,
Matrougui khalid
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a903-c
Subject(s) - medicine , cardiology
Type 2 diabetes is associated with vasculopathy. We this study we determined the nature of resistance artery structural remodeling in relationship with collagen type 1 content and stiffness in type 2 diabetes. Methods and Results: Blood glucose was high in diabetic compared to control mice. Freshly isolated mesenteric resistance arteries (MRA, 80–100 μm diameter) from type 2 diabetic db−/db− (diabetic) and non‐diabetic Db−/db+ (control) mounted in arteriograph and pressure‐passive diameter and wall thickness relationship were performed. Data indicated that MRA from diabetic mice have a smaller passive diameter than arteries from control under different level of pressure (25 to 125 mmHg). Measurement of wall thickness:lumen diameter ratio (25±1.8 vs. 15±1.2 at 75 mmHg diabetic vs. control respectively) and remodeling index (38±5% vs. control) revealed eutrophic remodeling of diabetic resistance artery. Mounted resistance artery from perfused mice with 4% of paraformaldehyde showed a small diameter and increased wall thickness strengthen our in vitro data. Mechanical properties of resistance artery revealed a great strain‐stress relationship in control vs. diabetic mice resistance arteries indicating increased stiffness. Western blot analysis showed increased collagen type 2 in diabetic compared to control mice resistance arteries in part responsible for structural remodeling and stiffness increase. Conclusion: These data indicate that type 2 diabetes, metabolic disease, is associated with eutrophic structural remodeling of mesenteric resistance artery with increased collagen type 1 content leading to stiffness enhance.