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The mRNA expression of eNOS, iNOS and L‐arginine transporters in the afferent arterioles (AA) of 2K1C hypertensive rats
Author(s) -
Hultström Michael,
Skogstrand Trude,
Helle Frank,
Iversen Bjarne Magnus
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a899-b
Subject(s) - enos , messenger rna , arginine , medicine , endocrinology , chemistry , kidney , afferent arterioles , nitric oxide synthase , nitric oxide , amino acid , blood pressure , biology , biochemistry , renin–angiotensin system , gene
Earlier investigations have shown that L‐NAME has a different effect on the contractile response to ANG II in the clipped (C) and the non‐clipped kidney (NC) from 2K1C animals due to increased NO release. The aim of the present study was to investigate mRNA expression of signalling molecules participating in NO release Hypertension was induced by a clip on the left renal artery. AAs were isolated 6 weeks after induction of hypertension by the iron oxide method. The cationic amino acid transferases (CAT1, CAT2 and CAT3), and the expression of NO synthetases in the AA (iNOS and eNOS) were studied by RT quantitative PCR related to 18S ribosomal RNA. Systolic BP was 127.5 ± 2.5 mmHg in sham operated rats and 142.8 ± 2.3 mmHg in 2K1C animals (p<0.05). The AA expression of mRNA for eNOS was 0.32 ± 0.04 in AA from C, 0.32 ± 0.07 in NC and 0.35 ± 0.02 in sham operated kidneys(>0.5). The mRNA for iNOS was not detectable in AA. The expression of CAT1 was 0.58 ± 0.07 in C, 0.59 ± 0.09 in NC and 0.32 ± 0.06 in the sham rats(p<0.01). Similar pattern for CAT2 was found. CAT3 was not detectable in the vasculature. In conclusion, the regulation of NO release in 2K1C was not dependent on changed expression of eNOS and iNOS. The increased release of NO may be explained by the increased mRNA expression for the L‐arginine transporters CAT‐1 and CAT‐2.