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Proteomic analysis of the renal medulla of Dahl salt‐sensitive rats and consomic SS‐13BN rats
Author(s) -
Tian Zhongmin,
Greene Andrew S.,
Usa Kristie,
Matus Isaac R.,
Pietrusz Jennifer L.,
Cowley Allen W.,
Liang Mingyu
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a896-b
Subject(s) - fumarase , microbiology and biotechnology , chemistry , western blot , isoelectric point , renal medulla , biochemistry , kidney , biology , gene , enzyme , endocrinology
The consomic SS‐13 BN rat, genetically identical to the Dahl salt‐sensitive (SS) rat except chromosome 13, exhibited a substantial reduction of blood pressure salt‐sensitivity. The present study utilized a proteomic approach to identify proteins that might contribute to the phenotypic differences between SS and SS‐13 BN . Differential in‐gel electrophoresis analysis of the renal medulla indicated that of approximately 2200 spots analyzed, 58 spots exhibited differential expression between 6‐week old SS and SS‐13 BN rats on a 0.4% NaCl diet (n=4, P<0.05, ratio >1.2 or <−1.2). MALDI‐TOF‐MS analysis successfully identified 42 spots, representing 28 unique proteins, 4 of which are encoded by genes located on chromosome 13. Western blot analysis confirmed the differential expression of 3 proteins (n=4, P<0.05) including fumarase (1.65 ± 0.12 fold higher in SS), heterogeneous nuclear ribonucleoprotein K (2.40 ± 0.32 fold higher in SS), and superoxide dismutase 1 (1.1 ± 0.03 fold higher in SS‐13 BN ). Moreover, fumarase, which catalyzes the inter‐conversion of fumarate and malate, exhibited a significant shift of isoelectric point toward the basic side in SS. The specific activity of fumarase was 1.89 ±0.14 fold lower in the renal medulla of SS (n=4, P<0.01). The present study provided new insights into the molecular basis for the phenotypic differences between SS and SS‐13 BN . (NIH R01HL077263 and N01‐HV‐28182)