Premium
Endothelial cell targeting of lipopolysaccharide‐induced brain inflammation
Author(s) -
Hirani Anjali,
Lee Won Hee,
Kang Sungkwon,
Lee Yong Woo
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a874-c
Subject(s) - inflammation , lipopolysaccharide , chemokine , cell adhesion molecule , antibody , immunology , tumor necrosis factor alpha , chemistry , medicine , pharmacology
Recent applications for nanotechnology in medicine are becoming one of the most innovative research areas. The potential for using nanoparticles conjugated with a specific ligand to target vascular endothelium, however, remains to be further investigated. The present study was designed to examine if immunotargeting of bioconjugated nanocrystals with a specific antibody against adhesion molecule ameliorates lipopolysaccharide (LPS)‐mediated brain inflammation. C57BL/6 mice were administered with a single intraperitoneal injection of LPS, followed by an intravenous injection of the bioconjugated nanoparticles. Brains were then isolated, and expression levels of pro‐inflammatory mediators were assessed by quantitative real‐time RT‐PCR. A significant up‐regulation of pro‐inflammatory cytokines (TNF‐α, IL‐1β, and IL‐6), chemokine (MCP‐1), and adhesion molecules (ICAM‐1 and E‐selectin) was observed in the mouse brain treated with LPS. In contrast, nanocrystals conjugated with E‐selectin antibody significantly and dramatically attenuated LPS‐induced overexpression of pro‐inflammatory mediators. However, the injection of same dose of E‐selectin antibody without nanocrystals did not affect LPS‐mediated brain inflammation. These data contribute to new opportunities for therapeutic exploration against brain inflammation (This work was supported by SBES Seed Grants).