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Intestinal microvascular responses to polymicrobial sepsis in prediabetic obese mice
Author(s) -
Singer Georg,
Terao Satoshi,
Stokes Karen Y,
Granger D. Neil
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a848-b
Subject(s) - sepsis , medicine , intravital microscopy , platelet , adiponectin , overweight , ligation , inflammation , endocrinology , obesity , microcirculation , insulin resistance
Sepsis is the leading cause of morbidity and mortality in non‐coronary intensive care units in the United States and has a death rate comparable to that of myocardial infarction. Obesity has been on the rise over the past few decades and currently nearly two thirds of US adults are overweight. There is growing evidence that obese individuals have a significantly higher mortality and complication rates in critically ill patients with sepsis. The objective of this study was to define the microvascular alterations elicited by cecal ligation and puncture (CLP), a model of sepsis, in two different mouse models (ob/ob & db/db) of obesity, compared to their lean counterparts. Intravital video microscopy was used to quantify leukocyte and platelet adhesion in the small bowel in wild type (WT) mice, ob/ob and db/db mice subjected to CLP. CLP (at 6 hrs) caused a significantly increased adhesion of leukocytes and platelets in both ob/ob and db/db mice compared to WT mice. No differences in blood cell adhesion were found in sham animals of all three strains tested. Adiponectin levels were significantly elevated in both sham and septic db/db mice when compared to WT mice. These findings indicate that obesity exaggerates the inflammatory and prothrombogenic phenotype that is assumed by the microvasculature during polymicrobial sepsis (Supported by DK43785).