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Physical training attenuates gene expression of ubiquitin‐proteasome pathway in diabetic mouse skeletal muscle
Author(s) -
Lehti Maarit,
Silvennoinen Mika,
Kivelä Riikka,
Kainulainen Heikki,
Komulainen Jyrki
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a837-c
Subject(s) - proteasome , skeletal muscle , ubiquitin ligase , endocrinology , ubiquitin , medicine , diabetes mellitus , muscle atrophy , messenger rna , gene expression , biology , gene , microbiology and biotechnology , biochemistry
It is known that Ub‐proteasome pathway is activated in skeletal muscle in atrophic conditions, like diabetes. We investigated the effects of physical training, diabetes, and their combination on Ub‐proteasome dependent proteolysis in skeletal muscle. Mice were divided to control (C), training (T), streptozotocin‐induced diabetic (D), and diabetic training (DT) groups. Training groups performed 1, 3, or 5 weeks of endurance training on a motor‐driven treadmill. Muscle samples from T and DT groups together with respective controls were collected 24 hours after the last training session. Total RNA was isolated from the left calf muscles and gene expression was analyzed with cDNA microarray. Diabetes induced changes in mRNA expression of the components of Ub‐proteosome pathway. mRNA level of proteins of the Ub‐conjugating complex, E2, were decreased while that of ubiquitin ligase (E3), proteasome 26S, and proteasome 20S were increased. Training alleviated diabetes‐induced changes in mRNA levels of proteasome 26S and 20S components. Induction of Ub‐proteasome pathway in diabetic skeletal muscle is in line with previous studies. Decreased expression of E2 may indicate regulative role for the step on the pathway. The results also suggest that endurance exercise is able to alleviate diabetes‐induced activation of Ub‐proteasome pathway that may indicate deceleration of skeletal muscle atrophy.