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Determinants of glucose tolerance in inbred mouse strains
Author(s) -
Berglund Eric,
Li Candice Y,
Ayala Julio E,
Powers Alvin C,
Wasserman David H
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a829-c
Subject(s) - medicine , insulin , endocrinology , clamp , chemistry , venous blood , mechanical engineering , clamping , engineering
Disposition index (DI) is an empirical measure of glucose tolerance integrating insulin sensitivity (SI) and secretion. To assess the strain dependence of DI, SI and insulin secretion were assessed at fixed hyperinsulinemic and hyperglycemic stimuli in 11wk old male mice of different strains using 2h insulin (ClampI) and hyperglycemic (ClampH) clamps. Arterial and venous catheters were implanted 1wk prior to study. Blood glucose (mg/dL) and insulin (ng/mL) in 5h fasted C57BL/6 (B6), 129X1/Sv (129), FVB/N (FVB), and DBA/2 (DBA) mice were 154±6, 123±4, 160±7, and 138±5 and 1.1±0.2, 0.6±0.1, 0.7±0.1, and 0.9±0.1. In ClampI (4 mU/kg/min), the glucose infusion rate (GIR; mg/kg/min) needed to clamp blood glucose at 150 was 54±2, 66±3, 57±3, and 46±2 in BL6, 129, FVB and DBA mice. SI [min −1 /(ng/mL)] in BL6, 129, FVB and DBA mice was 0.29±0.03, 0.29±0.05, 0.30±0.03, and 0.25±0.08. GIR needed during the ClampH to achieve blood glucose of 250 was 54±8, 65±11, 20±5, and 34±7 in BL6, 129, FVB, and DBA mice. Insulin AUC (ng/mL·min) was 126±21, 200±91, 5±6, and 106±31 in BL6, 129, FVB, and DBA mice and the DI was 37±0.8, 60±4.7, 1.7±0.2, and 26±2.5. Glucose disposal during ClampH was closely predicted by DI (r 2 =0.95), but not by SI (r 2 =0.2×10‐4). In conclusion, strain dependence in glucose tolerance is closely predicted by DI, but is not reflected in measures of insulin sensitivity alone.