Premium
Tissue hypoxia indicators after severe controlled hemorrhage in inbred rat strains
Author(s) -
Cortez D,
Cox A,
Bliss J,
Miranda N,
Ryan K L,
Kheirabadi B,
Klemcke H G
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a825-d
Subject(s) - inbred strain , basal (medicine) , hypoxia (environmental) , strain (injury) , blood lactate , analysis of variance , biology , medicine , endocrinology , andrology , chemistry , oxygen , biochemistry , heart rate , blood pressure , organic chemistry , gene , insulin
Eight‐fold differences in survival time were detected among 15 inbred rat strains after a controlled hemorrhage (Klemcke et al., FASEB J 20 : , 2006). To assess potential mechanisms of these differences, indicators of tissue hypoxia (lactate, pH, bicarbonate [HCO 3 ], and base deficit [BD]) were measured in arterial blood at the beginning (basal) and end (final) of hemorrhage. Rats were catheterized and, 24 hours later, 55% of the blood volume was removed during a 26 min period from conscious unrestrained animals. Rats were then observed for 6 hr or until death. Using average values for each strain, there were correlations (P <0 .01) between survival time and final lactate (r=−0.86), HCO 3 (r=0.88), and BD (r=−0.87), whereas associations between final pH and survival time were weak (r=0.5; P=0.05). There were no correlations between basal levels and survival time (P ≥0.16). In support of these correlations, analysis of all strains via ANOVA revealed that longer‐lived strains had lower (P<0.01) final lactate and BD values, but higher (P<0.01) final HCO 3 than strains with shorter survival times. Therefore, inbred rat strains demonstrate early differences in their ability to maintain aerobic metabolism that relate to their ability to survive severe hemorrhage.