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Phenotypic Changes and Gene Expression Profile in Hyperoxia‐Selected Drosophila melanogaster
Author(s) -
Zhou Dan,
Xue Jin,
Ali Sameh,
Zhao Huiwen W,
Dugan Laura,
Haddad Gabriel G
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a822
Subject(s) - hyperoxia , drosophila melanogaster , phenotype , gene , biology , genetics , gene expression , expression (computer science) , drosophila (subgenus) , melanogaster , medicine , computer science , lung , programming language
High O 2 levels, hyperoxia, can induce oxidative stress and affect many biological processes. In mammals, it has been shown that hyperoxia induces stress responses, inflammation and cell death through reactive oxygen spices (ROS). However, the genetic basis and molecular mechanisms underlying hyperoxic damage are still largely unknown. In the current study, we have generated a Drosophila melanogaster strain that can grow at extremely high O 2 (>90%), a lethal level for normal flies. Several phenotypic changes were found in the hyperoxia‐selected flies as compared to naïve ones. For instance, hyperoxia‐selected flies are bigger in body size and weight; they require longer time to recover from anoxia stupor; the oxygen consumption rate and ROS level were lower in the mitochondria isolated from the thorax muscle of the hyperoxia‐selected flies. In addition, gene expression profiling was performed using microarrays, and our data show that expression level of 284 genes were significantly altered (Fold change>1.50 and p<0.0001) with 94 up‐regulations and 190 down‐regulations. This hyperoxia‐selected stain provides a powerful tool to understand the genetic and molecular mechanisms that protect tissues and cells from oxidative injury.