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Effect of fatty acids on the production of reactive oxygen species (ROS) by J774 cells
Author(s) -
de Lima Thais Martins,
CuryBoaventura Maria Fernanda,
Gorjão Renata,
Curi Rui
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a815-a
Subject(s) - superoxide , chemistry , reactive oxygen species , hydrogen peroxide , arachidonic acid , biochemistry , oleic acid , linoleic acid , palmitic acid , fatty acid , enzyme
Several authors have shown modulation of macrophage functions by fatty acids (FA), including the production of cytokine, eicosanoids and reactive oxygen species. However, there is no report comparing the effect of the more abundant FA in plasma, palmitic (saturated) and oleic acids (monounsaturated, omega‐9), with omega‐3 (DHA and EPA) and omega‐6 (linoleic and arachidonic) FA on ROS production. The effect of different concentrations of these fatty acids on superoxide and hydrogen peroxide production by J774 cells (a model of murine macrophages) was investigated. The cells were treated with 25 to 100ìM of the fatty acids in the presence or not of 2.5ìg/mL of LPS and analysed by flow cytometry and colorimetric assay. The treatment was performed by 1 or 24 hours. All fatty acids, at 100ìM concentration or higher, increased superoxide production after 1 hour treatment. In the presence of LPS, this stimulatory effect was reduced. After 24 hours treatment, the effects of EPA and oleic acids on superoxide production were not observed. FA and LPS treatment for 24 hours did not induce any changes in superoxide production. All fatty acids increased hydrogen peroxide production in a dose dependent manner. In the presence of LPS, only oleic, linoleic, arachidonic and DHA acids enhanced LPS induced increase on hydrogen peroxide production. These findings indicate that all FA tested stimulate ROS production by J774 cells, but the effective concentration is specific for each one. Financial support: FAPESP, CNPq.