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The effect of Juglandis Semen extract on improvement of tBHT‐induced Na+‐pump inactivity in rabbit cerebral cortex
Author(s) -
Hong Yonggeun,
Lee Sujin,
Lee Sankil,
Kang Jaeseon,
Kim Jooheon
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a786-a
Subject(s) - chemistry , cerebral cortex , antioxidant , oxygen , ouabain , christian ministry , cortex (anatomy) , biochemistry , endocrinology , sodium , biology , philosophy , theology , organic chemistry , neuroscience
This study was undertaken to determine whether Juglandis Semen (JAS) extract exerts protective effect against oxidant‐induced inhibition of Na+‐pump activity in cerebral cortex. Na+‐pump activity was estimated by measuring ouabain‐sensitive oxygen consumption. The oxygen consumption significantly inhibited by 1 mM t‐butylhydroperoxide (tBHP), which was prevented by addition of 2% JAS extract. The oxygen consumption was increased by an increase in Na+ concentration from 5 to 100 mM, K+ concentration from 0.5 to 10 mM, and Mg2+ concentration from 0.2 to 5 mM. These changes in ion concentrations did not affect the inhibitory effect of tBHP and protective action of JAS on oxygen consumption. tBHP (1 mM) produced a significant increase in lipid peroxidation in cerebral cortex, which was prevented by 2% JAS extraction. These results suggest that JAS exerts protective effect against tBHP‐induced inhibition of Na+‐pump activity in the cerebral cortex, probably through action as antioxidant. This work was supported by the Research Project on the Production of Bio‐organs (No. 200508010801), Ministry of Agriculture and Forestry, Korea.