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Chromosome‐specific DNA repeat probes
Author(s) -
Weier H.Ulli G.,
Weier Jingly F.,
Baumgartner Adolf
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a774-b
Subject(s) - hybridization probe , fluorescence in situ hybridization , chromosome , computational biology , biology , nucleic acid , dna , in situ hybridization , molecular cloning , microbiology and biotechnology , genetics , gene , complementary dna , gene expression
Fluorescence in situ hybridization (FISH) has gained increasing popularity as a highly sensitive technique to study cytogenetic changes in the research environment as well as in the clinical laboratory. Today, hundreds of commercially available probes serve the basic needs of the biomedical research community. Widespread applications, however, are often limited by the lack of appropriately labeled, specific nucleic acid probes. We describe two rapid approaches to the preparation of chromosome‐specific probe DNAs and readily available methods to label the probes with the reporter molecules of choice. Notably, the techniques allow preparation of highly specific DNA repeat probes suitable for enumeration of chromosomes in interphase cell nuclei or tissue sections without a need for chromosome enrichment by flow cytometry and sorting or molecular cloning. Examples of production of DNA repeat probes specific for either human chromosome 17 or 18 also demonstrate that the entire process from probe concept and design to successful hybridization can be completed in the laboratory in just a few days without a need for highly specialized equipment.