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Supernate of packed red blood cells stored in AS‐5 additive solution accumulate mediators of inflammation during storage
Author(s) -
McFaul Steve J.,
Corley Jason B.,
Mester Craig W.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a773
Subject(s) - leukoreduction , platelet , packed red blood cells , flow cytometry , andrology , chemistry , red blood cell , immunology , inflammation , blood transfusion , medicine , biochemistry
Packed red blood cells (PRBC) are stored in additive solutions (AS) designed to support RBC integrity. It has been shown that PRBC stored in AS‐1 or AS‐3 accumulate pro‐inflammatory compounds during storage that may contribute to transfusion complications. The U.S. military stores PRBC in AS‐5, but PRBC in AS‐5 have not been evaluated for accumulation of pro‐inflammatory compounds. To evaluate PRBC stored in AS‐5, we collected twenty units of blood from healthy volunteers according to an IRB‐approved protocol, leukoreduced (LR) ten units, processed all units to PRBC with AS‐5, and stored the units at 4°C. Supernates of samples taken at various storage times were incubated with freshly collected blood at 37°C. Aliquots at 10 and 30 min were labeled with fluorochrome‐conjugated antibodies and assessed by flow cytometry for expression of CD11b on neutrophils (PMNs) and P‐selectin on platelets, and for formation of platelet‐PMN complexes. Supernates of non‐LR (NLR) PRBC stored for 42 days elevated side scatter and CD11b expression in PMNs by 14% and 130%, respectively, and increased the number of platelet‐PMN complexes by 20% compared to day 0 supernates. The magnitudes of these effects correlated with storage age. P‐selectin expression on platelets did not change. Pre‐storage leukoreduction abrogated these effects completely. Thus, NLR PRBC stored in AS‐5 accumulate pro‐inflammatory compounds, which is prevented by leukoreduction.