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Expression of MMP‐1, MMP‐9 and TGF‐β1 in Human Pterygium and Its Clinical Significance
Author(s) -
Ke Min,
Sun Zhong,
Wang Lin
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a766-b
Subject(s) - matrix metalloproteinase , pterygium , immunohistochemistry , extracellular matrix , conjunctiva , transforming growth factor , pathology , matrix (chemical analysis) , clinical significance , chemistry , biology , medicine , microbiology and biotechnology , ophthalmology , chromatography
OBJECTIVE: To investigate the expression of matrix metalloproteinase‐1 (MMP‐1), matrix metalloproteinase‐9 (MMP‐9) and transforming growth factor‐β1 (TGF‐β1) in human pterygium and explore its clinical significance. METHODS: 60 primary pterygium (experiment group) and 24 normal conjunctiva (control group) specimens were collected. Expression of MMP‐1, MMP‐9 and TGF‐β1 in two groups was detected using immunohistochemistry and RT‐PCR method. RESULTS: Immunohistochemistry result shows that the positive products of MMP‐1, MMP‐9 and TGF‐β1 can be found in all layers of pterygium, and overexpression in epithelium and hemoendothelial layer. The positive expression was stronger in experiment group than in control group. RT‐PCR result shows that expression level of MMP‐1 and MMP‐9 was higher in experiment group than in control one. The difference was significant (P <0.05) CONCLUSION: The overexpression of MMP‐1 and MMP‐9 in primary pteryguim may associate with degradation of extracellular matrix, dissolution of Bowman’s layer those results in invasion of cornea. The overexpression of TGF‐β1 may be related with hyperplasia of collagen fiber and accumulation of metamorphic fiber that lead to tissue apophysis.