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Myofibroblasts from skin wound and hypertrophic scar present different apoptotic response to body fluids
Author(s) -
Mayrand Dominique,
LopezVallé Carlos A,
Roy Michel,
Moulin Véronique
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a759-d
Subject(s) - myofibroblast , apoptosis , hypertrophic scar , wound healing , microbiology and biotechnology , pathology , chemistry , scars , fibroblast , biology , fibrosis , medicine , immunology , biochemistry , in vitro
Myofibroblasts play a central role in matrix formation and contraction during healing. At the end of healing, there is evidence that myofibroblasts disappear via apoptotic mechanisms. It has been postulated that a defect in myofibroblast apoptosis could be responsible for the formation of hypertrophic scars, but the stimuli that trigger apoptosis are unknown. Cells being surrounded by fluids containing molecules responsible of their cell fate, we have analyzed the cell apoptotic responses to serum and plasma as representative of body fluids. We have isolated and cultured human dermal fibroblasts (Fb), normal wound (Wmyo) and hypertrophic scar (Hmyo) myofibroblasts and compared their apoptotic rates in absence and presence of human serum and plasma, and fetal bovine sera. Using a propidium iodine, we have shown that presence of these body fluids induced a decrease of apoptosis in Hmyo and Fb, while an increase of apoptosis was denoted for Wmyo. These effects are at least due to thermally sensitive protein(s) with a molecular mass greater than 30 000D. These results confirmed the hypothesis of defects in apoptosis during pathological scar formation impeding myofibroblast disappearance. This study was granted by CIHR. VM was the recipient of scholarship from FRSQ.