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Expression and subcellular redistribution of claudins 6, 7 and 9 in human gastric adenocarcinoma
Author(s) -
Rendon Erika Patricia,
Fortoul T,
GarciaSamper X,
PiñonZarate G,
Cisneros L,
AlvarezFernandez G,
ZavalaZendejas V E,
Gorráez T,
Montaño L F
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a753-a
Gastric carcinoma is the second leading cause of cancer mortality worldwide and the fourth most common cancer diagnosed in the world. It has been established that malignant cells frequently exhibit abnormal tight junction structure and the loss of its integrity seems to be crucial for the diffusion of nutrients and other factors necessary for the growth and survival of the tumor cells. The expression of claudins has been found to be altered in several gastrointestinal tract cancers. Some authors has been reported changes in claudins 1, 3, 4 and 5 in the diffuse gastric carcinoma others state that claudins 1, 3, and 4 are strongly expressed in gastric intestinal‐type adenocarcinomas. The aim of our study was to evaluate the expression and the subcellular localization of other claudins in intestinal and diffuse type gastric adenocarcinomas from freshly obtained and paraffin‐embedded human tissue biopsies. Performing Western blot and immunohistochemical assays we detected expression of claudins 1, 2, 3, 4 and 5 in both diffuse and intestinal type carcinomas which correlate with previous reports. Interestingly our results also showed expression of claudins 6, 7 and 9 in intestinal‐type gastric adenocarcinoma; initial observations show that the expression of these claudins increases as the tumor progress to a more undifferentiated status. Supported by DGAPA IN225106