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In Vitro Fermentation Characteristics of Prebiotics by Feline and Canine Probiotic Bacterial Species
Author(s) -
Hussein Hussein S,
Kelly Michelle L.,
Boileau Thomas W.M.,
Sunvold Gregory D.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a745-d
Subject(s) - probiotic , prebiotic , fermentation , bifidobacterium , dry matter , food science , chemistry , bifidobacterium longum , completely randomized design , in vitro , lactobacillus , biology , zoology , bacteria , biochemistry , genetics
To determine the probiotic potential of feline ( Bifidobacterium longum [BF]) and canine ( B. pseudolongum [BC] and Lactobacillus murinus ruminis [LC]) colonic bacterial species (inocula), an in vitro study of 3 prebiotic substrates (fructooligosaccharides [FOS], galactooligosaccharides [GOS], and lactosucrose [LS]) and beet pulp (BP; a standard fiber in premium petfoods) was conducted. Thus, 240 serum bottles (125 mL; 192 for substrates and 48 for blanks) were used in a completely randomized design experiment with treatments being arranged as a 3 (inocula) × 4 (substrates) factorial. The bottles with (0.3 g dry matter [DM]) or without (blanks) substrates had 27 mL of anaerobic medium and 3 mL of inoculum (6 h growth in tryptic soy broth at 39°C) and were incubated at 39°C for 3, 6, 9, 12, 15, 18, 21, or 24 h (8 replications/treatment). Across substrates, extent of DM disappearance was similar (79.6%) among the 3 inocula but gas production was highest (P < 0.05) for BC (70.0 mL) and similar for BF and LC (31.1 mL). Lactate concentration was highest (P < 0.05) for LC (294.4 μg/mL) and similar for BF and BC (244.6 μg/mL). Across inocula, extent of DM disappearance was higher (P < 0.05) for prebiotics (99.1%) than for BP (20.9%) but gas production was lowest (P < 0.05) for BP (10.7 mL) and similar for the prebiotics (55.1 mL). Lactate concentration was highest (P < 0.05) for FOS and GOS (335.7 μg/mL), lowest (P < 0.05) for BP (110.1 μg/mL), and intermediate (P < 0.05) for LS (263.2 μg/mL). The results suggest LC to have the highest probiotic potential and both FOS and GOS to have the highest prebiotic potential.