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Effect of dietary intervention on serum C‐reactive protein and peripheral blood lymphocytes PPARs in dogs with chronic diarrhea
Author(s) -
Khoo Christina,
Gross Kathy L
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a745-a
Subject(s) - c reactive protein , medicine , diarrhea , crossover study , vitamin , inflammation , vitamin e , endocrinology , chemistry , antioxidant , biochemistry , pathology , alternative medicine , placebo
PPAR α and γ expressions have been shown in literature to ameliorate inflammation in animal colitis models and reduce C‐reactive protein, a marker for inflammation, in hyperlipidemic patients. The objective of this study was to determine the effect of dietary intervention with foods enriched with n3 fatty acids and antioxidants (vitamin E, C and beta carotene) on white blood cells PPAR γ and α and serum C‐reactive protein (CRP) in dogs with chronic diarrhea (CD). 10 healthy and 7 CD dogs were fed two balanced and complete wet adult dog foods (A and B) for 3 weeks each in a randomized crossover study. Both foods had similar n3 (0.2% DM) and slightly different n6 (1.0% vs 0.74 %) fatty acid levels and different levels of antioxidants, vitamin E (748 vs 166 mg/kg), vitamin C (336 vs 45 mg/kg) and ß carotene (1.3 vs 0.3 mg/kg) for Food A and Food B respectively. White blood cells PPAR α and γ were quantified by real time PCR and serum CRP by ELISA. Serum CRP was decreased in 100% of the CD dogs on Food A and only 57% of the CD dogs on Food B (p<0.05 by chi‐square analysis). PPAR γ was significantly higher in CD dogs (p=0.04) and was not affected by the food. PPARα tended to be higher in CD dogs and lowered by the n3 enriched food but this was not significant. The data showed that CD dogs have a different inflammatory profile than healthy dogs. Dietary intervention with n3 fatty acids and antioxidants can affect inflammatory biomarkers in these dogs.