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Effects of fructan supplementation on intestinal microbial populations of salmonella‐challenged weanling puppies
Author(s) -
Apanavicius Carolyn J.,
Vester Brittany M.,
KarrLilienthal Lisa K.,
Pope Lynda L.,
Fastinger Nathan D.,
Swanson Kelly S.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a745
Subject(s) - prebiotic , weanling , feces , inulin , salmonella , biology , bifidobacterium , cecum , temperature gradient gel electrophoresis , lactobacillus , food science , microbiology and biotechnology , probiotic , ileum , zoology , bacteria , biochemistry , fermentation , genetics , endocrinology , ecology , 16s ribosomal rna
Weanling puppies are at high risk for illness if exposed to Salmonella . Our objective was to evaluate the effects of fructan consumption on changes in fecal, colonic digesta, and ileal mucosa microbiota populations in weanling puppies challenged with Salmonella . After a 1 wk baseline period, 30 hound‐cross puppies (12 wk‐old) were randomly assigned to three diets containing: no prebiotic (control); 1% short‐chain fructooligosaccharides (scFOS); or 1% inulin. After 14 d on dietary treatment, dogs received an oral gavage of:S. typhimurium DT104 (5×108 CFU); or 0.9% saline (n = 5 per treatment). Fresh feces were collected at baseline and d 14. Colonic digesta and ileal mucosa samples were collected 48 h post‐infection. Following DNA extraction, denaturing gradient gel electrophoresis (DGGE) was used to generate microbial fingerprints and qPCR was used to quantify Bifidobacterium , Lactobacillus , C. perfringens , E. coli , and Salmonella concentrations. Microbial diversity was determined by calculating Dice’s coefficient. Data were analyzed using the mixed method of SAS. Although E. coli and C. perfringens were unchanged due to treatment, changes in Lactobacillus concentrations were greater (P < 0.05) in puppies fed inulin. It not known if this change contributed to the apparent protection from ileal mucosal damage that was observed in puppies fed inulin. Funding: USDA‐CSREES (Project #ILLU‐538‐396).