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Proteomics analysis reveal glucocorticoid pathway is down‐regulated in canine chronic diarrhea
Author(s) -
Khoo Christina,
Li Junyu,
Gross Kathy L,
AlMurrani Samer
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a744-b
Subject(s) - inflammation , diarrhea , glucocorticoid , proteomics , downregulation and upregulation , medicine , immunology , biology , biochemistry , gene
Chronic diarrhea in animals is a condition that can involve inflammation of the intestinal tract and is treated with dietary intervention, antimicrobial medication and immunosuppressive therapy. The goal of this study was to identify pathways that are perturbed in dogs with chronic diarrhea using proteomics to determine the appropriate intervention. In this study, 10 healthy and 7 dogs with naturally occurring chronic diarrhea (CD) were fed a complete and balanced adult dog food for 6 weeks. At the end of 4 weeks, plasma samples were collected and analyzed using 2D gel electrophoresis and LC/MS/MS. Prior to the analysis, the plasma samples were treated using the Ciphergen Equalizer ™ beads service to equalize the protein populations. Eight proteins were differentially expressed between healthy and CD dogs (p <0.05). Six proteins were down‐regulated and two proteins were up‐regulated in CD dogs by approximate 1.5‐7 fold. The majority of the proteins are involved in the glucocorticoid response pathways, while others are in the coagulation cascade and lipoprotein metabolism pathways. These results support the current literature on human and rodent gastrointestinal inflammation that described downregulation of the glucocorticoid response suggesting that alleviating inflammation is a target for the chronic diarrhea condition.