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Bioavailability of tea catechins and their effect on gene expression in dogs
Author(s) -
PaetauRobinson Inke,
Locniskar Mary F.,
Gao Xiangming,
AlMurrani Samer
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a744
Subject(s) - catechin , bioavailability , epigallocatechin gallate , green tea , ingestion , green tea extract , gene expression , food science , meal , white blood cell , camellia sinensis , epicatechin gallate , biology , pharmacology , chemistry , gene , endocrinology , biochemistry , medicine , polyphenol , botany , antioxidant
Green tea is believed to positively affect weight management, risk of cardiovascular disease, and risk of cancer. In this study we determined the absorption of catechins from green tea and their effect on gene expression in white blood cells in dogs. Eighteen Beagles (2 – 9 yrs), randomized to two treatment groups, participated in the 1‐day study. The dogs either received the control diet or the control diet plus green tea extract. The green tea was added to the meal prior to feeding and provided 76 mg epicatechins/kg body weight. Blood was collected prior to feeding, and at hour 1.5, 2.5, 6, and 24 post‐feeding. Plasma catechins were analyzed at all time points. White blood cells were isolated from the blood samples collected prior to feeding, and at 6 and 24 hours post‐feeding for gene expression analysis. The peak plasma catechin concentrations achieved by feeding green tea occurred between 1.5 hr and 2.5 hr post‐feeding and were 31 μg/L, 23 μg/L, 98 μg/L, and 59 μg/L for epicatechin, epicatechin gallate, epigallocatechin, and epigallocatechin gallate, respectively. Following ingestion of green tea, 560 genes were differentially expressed including genes that are associated with lipid metabolism, cell cycle regulation, and cancer. We can conclude that tea catechins are absorbed from green tea extract in dogs. Based on gene expression data green tea has an effect on lipid metabolism and cell cycle regulation.