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Effect of a dietary antioxidant package on immune function and antioxidant status of IBD cats.
Author(s) -
Khoo Christina,
PaetauRobinson Inke,
Gross Kathy L
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a742-c
Subject(s) - cats , immune system , antioxidant , medicine , vitamin e , lymphocyte , vitamin c , endocrinology , comet assay , vitamin , immunology , dna damage , chemistry , biochemistry , dna
The objective of this study is to evaluate the effect of antioxidants on immune and inflammatory parameters in cats with inflammatory bowel disease (IBD). In this study, 10 healthy and 10 IBD cats were fed wet food with low antioxidants (CTRL) and test food (AOX) with added vitamin E, vitamin C and beta carotene (85.2 vs 235.6 IU, 35.4 vs 87.3 μg/g, 0.05 vs 0.92 μg/g respectively) for 4 weeks each in a randomized cross‐over design. Both foods were completely balanced for adult cats. Serum vitamin E and C, DNA damage (comet assay), lymphocyte subsets and proliferation were measured at weeks 4 and 8. Real time PCR was used to determine cytokine levels from stimulated lymphocytes ex vivo. The results showed that serum vitamin E and C were significantly increased in healthy (972.9 vs 775.4 μmol/L, 215.8 vs 164.7 μmol/L) and IBD cats (833.6 vs 636.2, 232.8 vs 159.0 μmol/L) on the AOX vs CTRL food (p<0.05). IBD cats had significantly higher lymphocyte proliferative activity when stimulated with ConA (1.63 vs 1.24, p<0.05) and PWM (1.21 vs 1.06, p=0.054) and higher CD4+ T helper cells compared to the healthy cats (p<0.05). In contrast, stimulated lymphocytes from IBD cats produced significantly lower levels of IL1ß and IL2 than those from healthy cats (p<0.05). The AOX food tended to reduce the lymphocyte proliferation activity in both groups of cats. The results showed that IBD cats have a dysregulated and hyper‐inflammatory immune response compared to healthy cats. Higher levels of antioxidants or nutrients such as fish oil may be indicated for decreasing inflammation.