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Effects of flaxseed lignan on mitogen stimulated lymphocyte proliferation
Author(s) -
Rhee Yeong,
Brunt Ardith
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a737-a
Subject(s) - concanavalin a , lignan , incubation , lymphocyte , thymidine , incubation period , chemistry , whole blood , medicine , immune system , in vitro , endocrinology , immunology , biology , microbiology and biotechnology , andrology , biochemistry , stereochemistry
This study determined the effects of flaxseed lignan, s ecoisolariciresinol diglycoside (SDG), on in vitro lymphocyte reactivity to mitogens. Twelve‐hour fasting blood samples were collected four times from a healthy donor. Heparinized whole blood was diluted with complete RPMI, and then incubated with 0, 10, 50, or 100 μM SDG/ml for 4 hrs at 37 °C. Upon completion of the incubation, whole blood cells were incubated with 0, 10, 20, 40, or 80 μg/ml phytohemagglutinin–L (PHA‐L) or 0, 12.5, 25, 50, or 100 μg/ml concanavalin A (ConA) for 96 hours at 37 °C. Eighteen hours before the end of the incubation, blood cells were pulsed with 1 μCi 3 H‐thymidine. The 3 H‐thymidine uptake by lymphocytes was counted and reported as DPM. Both PHA‐L and ConA significantly increased lymphocyte proliferation (p<0.0001). A higher lymphocyte proliferation was observed with a higher concentration of mitogens; however, the lymphocyte proliferation was higher with PHA‐L when compared to ConA. No significant effects of SDG on lymphocyte proliferation were observed. The results of this study indicate that flaxseed lignan has no effects on the immune system. Funding support: NDSU HDE College, Swenson Endowment, and ND EPSCoR; equipment support: USDA NRI CSREES #2005‐35200‐15258.