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Epo effect on muscle cells growth and differentiation is related to O2 environment
Author(s) -
michèle Beaudry,
Séverine Divoux,
Alain Duvallet,
Patricia Quidu,
Fabrice Favret,
JeanPaul Richalet,
Thierry Launay
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1426-b
Subject(s) - erythropoietin , myocyte , cell culture , cell growth , erythropoietin receptor , endocrinology , cellular differentiation , microbiology and biotechnology , medicine , receptor , biology , chemistry , biochemistry , gene , genetics
Besides its classical role in the regulation of the red blood cell mass, erythropoietin (Epo) has recently been described for biological effects in various cells and tissues. Based on only one work Epo has been described to enhance murin myoblast proliferation and reduce differentiation. The aim of our work was to use these properties in order to increase the accumulation of myogenic cells in human primary myoblast cultures. Our results gave evidence for the expression of erythropoietin receptor (EpoR) in human cultured myoblasts and murine cells from the L6 line but no effect was observed on myoblast proliferation and differentiation in standard experimental conditions of culture (21% O 2 and DMEM with 4.5g/l glucose), contrary to previous work. When muscle cells were cultured in 1 % O 2 and DMEM with 1g/l glucose a strong increase of cell proliferation was observed with 2u/ml Epo. In these conditions of culture non treated myoblast growth was decreased. However, in both treated and non treated muscle cells grown in these conditions (1 % O 2 and DMEM with 1g/l glucose) differentiation was dramatically inhibited. This study should be of great interest in muscle pathology cellular therapies. Support by Association Française contre les Myopathies (AFM)