Premium
Degranulation of mast cells (MC) and potential role of MC‐derived interleukin‐8 (IL‐8) in ovarian follicle growth and ovulation – an in vitro study
Author(s) -
Szukiewicz Dariusz,
Maslinska Danuta,
Klimkiewicz Jakub
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1422-a
IL‐8 can be considered an important regulatory factor in the ovary. Number of IL‐8 positive cells is subjected to changes during the estrus and corresponds with ovarian MC. In this two‐step in vitro study we examined: Influence of IL‐8 on the development and ovulation of ovarian follicles; Correlation between MC degranulation and IL‐8 concentration in follicular fluid (FF). For the 1st step of the study, preantral follicles (N=120) were cultured individually. Diameter of the follicles was measured daily. Starting from Day 3, the medium was supplemented with IL‐8 or IL‐8 and blocker of IL‐8 receptors CXCR1/R2 (repertaxin). At the end of culture period, the follicles were induced to ovulate with hCG. In the 2nd step, murine ovarian explants (N=60) were cultured for six days on membrane inserts. The medium was supplemented with compound 48/80 and concentrations of IL‐8 in FF were measured by ELISA. We found, that: Mean daily increase in follicular size as well as the mean ovulation rate was higher after administration of IL‐8 (p <0.05). Compound 48/80 produced significant increase in IL‐8 concentration in FF. Our results indicate influence of IL‐8 on ovarian follicle growth and ovulation as well as potential involvement of MC in these processes. Supported by AM grant: 2M2‐W2‐06.