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The role of estrogen on the development of high fat diet induced metabolic syndrome
Author(s) -
RomeroAleshire Melissa Jill,
Keck Maggie,
Hoyer Patricia B,
Brooks Heddwen L
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1416-a
Subject(s) - endocrinology , medicine , menopause , metabolic syndrome , estrogen , dyslipidemia , hormone , insulin resistance , insulin , obesity
The impact of estrogen on the development of the characteristics of the metabolic syndrome is not well studied. High fat diet is commonly used to induce metabolic syndrome in mice. The aim of this study was to determine the impact of menopause on the progression of metabolic syndrome. We used the 4‐vinylcyclohexene diepoxide (VCD) model of menopause, which mimics both the perimenopausal period and the postmenopausal (PO) ovarian production of androgens. We fed VCD‐treated female mice a high fat diet (HFD). After 12 weeks of HFD treatment, PO mice had higher fasting blood glucose than cycling control mice (143 ± 11 v 96 ± 6 mg/dL, p<0.05) and had impaired glucose tolerance. After 16 weeks of HFD both PO and control mice had elevated circulating levels of insulin (1112ng/ml in PO; 621ng/ml in control, p<0.05) and exhibited dyslipidemia. At the end of the study kidneys were processed for real‐time PCR or fixed for immunohistochemistry. βENaC, collagen 1α1 and 1α2 mRNA were significantly increased in renal cortex of PO mice compared to cycling controls on a HFD. These results suggest that hormonal changes during menopause promote the development and progression of metabolic syndrome in mice fed a high fat diet.