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Characterization of thromboxane receptor expression in cultured sensory neurons
Author(s) -
Howard Tessa E.,
Gilbert William J.,
Hanke Justin J.,
Wacker Michael J.,
Orr James A.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1407-b
Subject(s) - trpv1 , capsaicin , nociception , dorsal root ganglion , receptor , nodose ganglion , chemistry , sensory system , resiniferatoxin , sensory neuron , medicine , endocrinology , anatomy , neuroscience , biology , vagus nerve , transient receptor potential channel , stimulation
Our laboratory has previously reported that thromboxane A2 (TxA2) stimulates cardiac vagal sensory neurons and elicits reflex changes in arterial blood pressure and heart rate. The purpose of this study was to characterize the expression of TP in nodose ganglion (NG) and dorsal root ganglion (DRG) neurons. Since the vanilloid type 1 receptor (TRPV1), also known as the capsaicin receptor, is expressed in nociceptive neurons and TxA2 is released during tissue trauma, we hypothesized that TxA2 may be preferentially expressed in nociceptive neurons. These experiments were carried out using multiplex single‐cell RT‐PCR on cultured NG and DRG neurons from the rabbit. Single cells were isolated and placed in RT‐PCR buffer containing primers for neurofilament medium (NFM), the capsaicin receptor (TRPV1), and the TxA2 receptor (TP). NFM was used as a positive neuronal marker, while TRPV1 was used to distinguish nociceptive versus non‐nociceptive neurons. 66.3% and 24.7% of NFM positive NG neurons were positive for TRPV1 and TP, respectively (n=89). In the DRG, 83.8% and 13.1% of NFM positive neurons were positive for TRPV1 and TP, respectively (n=99). However, TP was not preferentially expressed in nociceptive (TRPV1 positive) neurons or neurons with small cell bodies. (Howard was supported by the KU Self Fellowship REU Program and the Dept. of Molecular Biosciences; Gilbert by NIGMS R25 62881; Hanke by NIGMS R25 GM62232)