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Dysregulated Expression Of Renal Epithelial Sodium Channels (ENaC) In Dahl Rats.
Author(s) -
Amin Md Shahrier,
Reza Erona,
Wang HongWei,
Leenen Frans H H
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1406-c
Subject(s) - epithelial sodium channel , messenger rna , endocrinology , medicine , kidney , chemistry , immunohistochemistry , renal medulla , sodium , staining , medulla , microbiology and biotechnology , protein expression , biology , biochemistry , gene , organic chemistry , genetics
ENaC plays an important role in Na transport. Effects of high salt diet on α, β and γ ENaC expression in Dahl S vs R rats has not yet been studied. S and R rats were fed regular [ rs , 0.3%] or high [ hs , 8%] salt for 2 weeks. mRNA and protein expression in kidney were studied by qRT‐PCR and immunoblotting and cellular distribution assessed by immunohistochemistry. In cortex, hs increased β and γ mRNA and protein in R but decreased β protein in S. γ protein was lower in S than R. Cellular distribution was not affected.In medulla, ENaC mRNA abundance was similar in S and R on rs;hs increased β and γ mRNA but not protein in R medulla and decreased α protein abundance in inner medulla (IM). Protein abundance in S was ~2fold higher than R: for α and β in outer medulla (OM) and for γ in IM. Apical staining of all subunits was more in IM collecting ducts of S than R. 2 weeks hs further increased γ protein and apical staining in IM of S. These results indicate strain dependent regional ENaC regulation by hs . S appear to have greater ENaC abundance in IM which might contribute to Na retention.