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Enzymes of L‐arginine metabolism and their regional induction in the heart following myocardial infarction
Author(s) -
Stayton Mark M,
Keele Jacque A,
Harpster Mark H,
Bandyopadhyay Somnath,
McCormick Richard J,
Thomas D. Paul
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.6.a1404-c
Subject(s) - arginase , ornithine decarboxylase antizyme , arginine , ornithine decarboxylase , nitric oxide synthase , ornithine , nitric oxide , enzyme , arginine decarboxylase , western blot , biosynthesis , ornithine aminotransferase , ventricle , chemistry , biochemistry , microbiology and biotechnology , medicine , endocrinology , biology , amino acid , gene
l ‐Arginase 1 (ARG1) is the single most highly induced transcript in the mouse left ventricle after acute myocardial infarction (AMI). Within ischemic/infarcted tissue (IF), the ARG1 transcript is induced 121‐fold by 24 h post‐AMI. In addition to induction of the ARG1 transcript, arginase specific activity is elevated 34‐fold at 48 h increasing to 42‐fold by 1‐week. The l ‐arginase 2 (ARG2) transcript is also induced along with transcripts encoding enzymes of polyamine biosynthesis and a protein inhibitor of nitric oxide synthase (NOS) activity. Thus, nitric oxide production may be regulated, in part, by inhibition of NOS and depletion of the NOS substrate, l ‐arginine, by l ‐arginase. In addition, l ‐arginine may undergo conversion to polyamines as part of the stress response and/or to proline (collagen biosynthesis). To test these hypotheses, we produced antibodies against ARG1, ornithine decarboxylase, antizyme inhibitor and protein‐inhibitor of NOS. As judged by western blot and immunohistochemical analyses, ARG1 and the antizyme inhibitor polypeptides show induction in the IF. We have further evaluated the regional and cell‐type specificity of their induction by AMI within the mouse left ventricle. Support: NIH grants P20 RR15640 and R15 HL71239